Does the number of variants matter?

I can do a sample size calculator but I'm not sure if that just works for simple A/B testing.

For example, we have 100k email list.

Using this tool:, my sample size is 6373 for 90% confidence with 1% margin of error.

Does that mean I send 6373x5 or does that number only work for a 50/50 send?

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