Researchers from The Novo Nordisk Foundation Center for Biosustainability at Technical University of Denmark (DTU), Chalmers University of Technology and KTH Royal Institute of Technology have identified 9 gene targets which upon combinatorial silencing improve protein production in engineered yeast cells by 2.2-fold.
"The concept can be extended to other yeast protein producers, even some filamentous fungi and mammalian cell factories.
Any organization that works with superior protein producers can use these findings," says first-author Postdoc Guokun Wang from The Novo Nordisk Foundation Center for Biosustainability at DTU.
The method was used to improve the yeast's production of α-amylase - a model protein which indicates overall production values of sought-for proteins (recombinant proteins) in the cell.
Silencing is a powerful tool
The optimized yeast strain was achieved by determining several gene targets suitable for silencing via RNA interference (RNAi).