What are exosomes?
Exosomes, a tiny membrane vesicle with a lipid bilayer structure, are secreted by most cells and are approximately 40-200 nm in diameter. exosomes are found in body fluids, including blood, saliva, urine and breast milk. exosomes of different tissue origins differ in content composition and function, and this difference is dynamically regulated by the extracellular matrix and microenvironment. Exosomes secreted by host cells or tumor cells are involved in tumorigenesis, growth, invasion and metastasis. The analysis and detection of exosomes can assist in early diagnosis, efficacy evaluation and prognostic analysis of the disease.
Exosome isolation and extraction method
For exosome research, isolation and collection of exosomes is a very critical step. There are several main methods for exosome collection: ultra-high speed centrifugation, density gradient centrifugation, magnetic bead immunization, ultrafiltration method, polymer precipitation, etc., and each method has different advantages and disadvantages. After extraction, exosomes need to be identified. Currently, electron microscopy techniques (morphological identification), NTA (particle size analysis), and Western blot assay (detection of marker proteins, such as CD9, CD63, CD81, TSG101, HSP70, etc.) are commonly used to identify exosomes.
Exosome proteomics research
Exosomes can affect the function of target cells by transmitting messages, activate cellular signaling pathways, and play a role in immune, coagulation, tumor and other physiopathological processes. And the differential expression of proteins among them is an important feature to distinguish exosomes of different origins and is an important way to study the origin of exosomes. Proteomics has gradually become an important tool for studying the proteomes of various subcellular fractions. Proteomics studies on exosomes can reveal the pathogenesis of diseases, search for biomarkers for disease diagnosis and prognosis, and screen for disease therapeutic targets.
Creative Proteomics offers differentiated exosome proteomics services based on the different needs of researchers.
1. Tandem mass tag (TMT) quantitative proteomics
TMT is a classic high-throughput screening technique in quantitative proteomics that uses 6, 10 and 16 isotopes of labels to specifically label amino groups of peptides for tandem analysis by high-resolution mass spectrometry, allowing simultaneous comparison of protein expression between up to 16 samples.
2. Label free proteomics
Label free protein quantification allows the relative quantification of peptides by mass spectrometric analysis of enzymatically cleaved peptides without the use of expensive stable isotope labels as internal standards, simply by analyzing the mass spectrometric data generated during large-scale protein identification and comparing the signal intensity of the corresponding peptides in different samples.
COVID-19 Impact Analysis on Exosome Technologies Market report published by Value Market Research provides a detailed market analysis comprising market size, share, value, growth and trends for the period 2019-2027.
The report encompasses data regarding market share and recent developments by key players.
Moreover, this market report also covers regional and country markets in detail.The research report also covers the comprehensive profiles of the key players in the market and an in-depth view of the competitive landscape worldwide.
The major players in the Exosome Technologies Market include Aegle Therapeutics, Aruna Biomedical, Evox Therapeutics, ExonanoRNA LLC., Anjarium Biosciences, Carmine Therapeutics, and Exosome Diagnostics, Inc..
This section includes a holistic view of the competitive landscape that includes various strategic developments such as key mergers & acquisitions, future capacities, partnerships, financial overviews, collaborations, new product developments, new product launches, and other developments.Get more information on "Global Exosome Technologies Market Research Report" by requesting FREE Sample Copy at https://www.valuemarketresearch.com/contact/exosome-technologies-market/download-sampleMarket SegmentationThe broad Exosome Technologies Market has been sub-grouped into the Exosome Type, Cell Source, Indication , End-user and Region.
The report studies these subsets with respect to the geographical segmentation.
