1. Methodology to be considered for air displacement pipetting
If you are going with air displacement then do reverse pipetting. This technique results in the sample volume plus an additional volume being aspirated into the tip. It can be achieved by pressing to the first stop to expel only the target sample volume. Forward mode pipetting is not recommended, as it often leaves a residual volume of undelivered sample in the tip.
2. Use Appropriate pipette tip
3. Immerse the Tip to a Proper depth and hold vertically while Aspiration
Immersion depth should be in track to the liquid volume
4. Reduce Plunger speed and pause sufficiently after aspiration and dispense
The appropriate pause duration should be determined at the beginning and kept consistent each time. To determine whether the pause after aspiration is long enough, examine the filled tip after removing from solution. If a trailing air gap forms, a longer pause needed. After dispensing, examine the bottom of the pipette tip. If a droplet forms, a longer pause needed.
5. Avoid Air bubbles
Air bubbles in viscous solution takes long time to dissipate and so can reduce the volume accuracy. It is highly recommended not to mix instead, Use an orbital shaker or centrifuge to remove existing bubbles.
About Accumax
Accumax is a global manufacturer and supplier of lab equipments. We manufacture more than 200000 pipettes annually, cater to customers in over 130 countries around the globe.
We are certified with ISO 9001-2015 & ISO 13485-2016 and we strictly maintained stringent quality controls to ensure that the end-product reaching the customer is precisely calibrated and satiates the needs of our customers.
Original Source: https://accumaximum.com/blogs/tips-to-improve-pipetting-of-viscous-solution/
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