A Fluorescence Spectrophotometer is a scientific instrument used to measure the fluorescence emission of a sample. It is widely employed in various fields such as biochemistry, molecular biology, pharmaceuticals, environmental science, and materials science. This instrument operates based on the principle of fluorescence, which involves the absorption of light at a specific wavelength by a molecule and the subsequent emission of light at a longer wavelength.
The main components of a Fluorescence Spectrophotometer typically include:
Light Source: A high-intensity lamp or a laser that emits light at specific wavelengths, usually in the ultraviolet (UV) or visible range. The light source is selected based on the excitation wavelength required for the sample being analyzed.
Monochromators: These are used to select the excitation and emission wavelengths. A monochromator consists of a diffraction grating or prism that disperses the incoming light into its component wavelengths, allowing the user to select a specific wavelength for excitation and detection.
Sample Compartment: A space where the sample is placed for analysis. It is designed to minimize stray light and external interference. The sample is usually contained in a cuvette, a transparent container made of glass or quartz.
Optics: A series of lenses and mirrors that focus and direct the light onto the sample and collect the emitted fluorescence signal. These optical components ensure efficient excitation and detection of fluorescence emission.
