In the realm of research and drug development, cell lines have become a widely used model for studying both healthy and diseased tissues. Their ease of handling, cost-effectiveness, and stability have made them an attractive choice. However, it is crucial to recognize that cell lines might not fully mirror the complexity of in vivo situations. For researchers aiming to model intricate physiological behaviour, human primary cells offer a more suitable option.
Imagine planting a flower garden where you have the choice of selecting expensive and high-maintenance flowers that perfectly fit your environment or opting for affordable varieties that grow everywhere but lack perfection. Similarly, when researchers must decide between primary cells and immortal cell lines for their projects, several factors come into play.
If your goal is to build a cell culture model that closely mimics human in vivo conditions, primary cells should be your preference. Additionally, for publishing results, validating them in a system relevant to human physiology is advisable. Conversely, if you seek a model that is convenient, well-established, and easy to work with, immortal cell lines are the preferred option, even though their behavior might not precisely resemble that of human cells.
Peeking into Real Life with Human Primary Cells
Human primary cells are directly isolated from tissues, retaining the morphological and functional characteristics of their tissue of origin. For instance, original tumour tissue from colorectal cancer preserves numerous tumour markers and known microRNAs, while cell lines display differences in their expression.
However, primary cells have a limited lifespan as they undergo senescence processes, self-renewal potential, and differentiation capabilities. It is best to use them in early passages to avoid morphological and functional changes that occur with ageing. The genetic characteristics and age of donors are also influential factors, as the cells' behaviour can vary under the same culture conditions. This variability can be useful when investigating cellular features across populations or testing new compounds. However, if low variance is required, using cells from the same donor is recommended.
Primary cells offer the advantage of obviating the need for animal models, minimizing inter-species differences that could impact drug toxicity and mode of action.
Handling Primary Cells with Care
Primary cell cultures can originate from healthy or cancerous cells, sourced from organ donations, surgical specimens, fetal tissues, or post-mortem donors. However, the limited availability of human primary cell sources means that obtaining extra material from the same donor might not be possible. Additionally, derived from tissue explants consist of a mix of different cells at different stages, necessitating the purification of specific cell types for more homogeneous cultures. Due to their sensitivity, human primary cells often require additional nutrients and growth factors for optimal growth.
It is crucial to optimize culture conditions for each cell type, as different cells have distinct media requirements. Notably, high variability of serum content can influence research results when using cell lines. High levels of antibiotics can also negatively affect the viability and growth of primary cells. To ensure quality control, thorough characterization of cells at different stages of culture is essential, and adherence to good cell culture practices, including comprehensive documentation, ensures model transparency and reproducibility.
Cell Lines: Vital Tools in Biological Research
The use of primary cells is built upon decades of research involving cell lines. Since the early 20th century, cell lines have provided valuable insights into the biological processes of human cells. Immortal cell lines serve as powerful tools for various applications, including drug metabolism and cytotoxicity testing, gene function studies, and the production of vaccines, antibodies, and biological compounds.
Choosing between primary cells and immortal cell lines depends on the research objectives. For investigations into basic biological processes, cellular function manipulation, new method establishment, or preliminary screenings, immortalized cell lines are favoured for their cost-effectiveness, ease of handling, and extended culture capabilities. Furthermore, high throughput screening can benefit the unlimited supply of material provided by cell lines.
However, it is essential to acknowledge that while experimental findings obtained from cell lines can be readily interpreted and compared, their physiological relevance may be limited. Serial passages can introduce genotype and phenotype variations, resulting in altered genomic content and abnormal expression profiles. Not possessing key morphological or functional features, cell lines might not induce relevant biomarkers. Consequently, findings from cell lines may not be directly translatable to human systems. Moreover, the prolonged use of immortal cell lines can lead to contamination by other cells or microorganisms, necessitating thorough validation to avoid misidentification or contamination.
In conclusion, the selection between primary cells and immortal cell lines should align with the research aim. Immortal cell lines serve as valuable models for preliminary experiments, while primary cells offer the opportunity to replicate and confirm key findings, enhancing the translational potential and relevance to human physiology.
According to the new market research report “Primary Cells Market by Origin (Human Primary Cells, Animal Primary Cells), Type (Hematopoietic, Dermatocytes, Gastrointestinal, Hepatocytes, Lung, Renal, Musculoskeletal, Heart), End User, Region – Global Forecast to 2025″, published by MarketsandMarkets™, the market is projected to reach USD 1,613 million by 2025 from USD 970 million in 2020, at a CAGR of 10.7%Browse and in-depth TOC on “Primary Cells Market”108 – Tables34 – Figures179 – PagesDownload PDF Brochure: https://www.marketsandmarkets.com/pdfdownloadNew.asp?id=32854960The Growth in this market is largely driven by the rising prevalence of cancer, increasing focus on the development of novel cancer therapies, and rising adoption of primary cells over cell lines.
Emerging economies such as China and Japan are providing lucrative opportunities for the players operating in the Primary Cells Market.The human primary cells segment accounted for the largest share of the Primary Cells Market, by origin segment, in 2019Based on origin, the Primary Cells Market is segmented into human and animal primary cells.
The human primary cells segment accounted for the largest share in the Primary Cells Market in 2019.
The growing application areas of human stem cells and the rising incidence of cancer are the major factors driving the growth of this segment.Hepatocytes segment to register the highest growth rate during the forecast periodBased on type, the Primary Cells Market is segmented into hematopoietic cells, dermatocytes, gastrointestinal cells, hepatocytes, lung cells, renal cells, heart cells, musculoskeletal cells, and other primary cells.
Increasing cancer research in life science research companies, the increasing number of R facilities, high adoption of primary cells in cell-based experiments, and the increasing investments of companies in cell-based research are the major factors driving the growth of this segment.Request Sample Pages : https://www.marketsandmarkets.com/requestsampleNew.asp?id=32854960North America is the largest regional market for Primary Cells MarketThe Primary Cells Market is segmented into four major regions, namely, North America, Europe, Asia Pacific, and the Rest of the World (RoW).
The growth in the North American Primary Cells Market can be attributed to increasing funding for cancer research, growing life science research sector, expansion of the healthcare sector, and the high adoption of stem cell therapy & cell immunotherapies for the treatment of cancer and chronic diseases.The major players operating in Primary Cells Market are Thermo Fisher Scientific, Inc. (US), Merck KGaA (Germany), Lonza (Switzerland), Cell Biologics, Inc. (US), PromoCell GmbH (Germany), HemaCare Corporation (US), ZenBio, Inc. (US), STEMCELL Technologies, Inc. (Canada), Corning Incorporated (US), AllCells (US), American Type Culture Collection (US), Axol Bioscience Ltd. (UK), iXCells Biotechnologies (US), Neuromics (US), StemExpress (US), BioIVT (US), ScienCell Research Laboratories, Inc. (US), PPA Research Group, Inc. (US), Creative Bioarray (US), BPS Bioscience, Inc. (US), Epithelix Sàrl (Switzerland), ReachBio LLC (US), AcceGen (US), Sekisui XenoTech, LLC (US), and Biopredic International (France).Browse Adjacent Markets @ Biotechnology Market Research Reports & ConsultingBrowse Related Reports:3D Cell Culture Market by Product (Hydrogel, Hanging Drop, Bioreactor, Microfluidics, Magnetic Levitation), Application (Cancer, Stem Cell, Toxicology, Tissue Engineering), End User (Pharmaceutical, Biotech, Cosmetics), Region – Global Forecast to 2024https://www.marketsandmarkets.com/Market-Reports/3d-cell-culture-market-191072847.htmlCell Expansion Market by Product (Reagent, Media, Flow Cytometer, Centrifuge, Bioreactor), Cell Type (Human, Animal), Application (Regenerative Medicine & Stem Cell Research, Cancer & Cell-based Research), End-User, and Region – Global Forecast to 2025https://www.marketsandmarkets.com/Market-Reports/cell-expansion-market-194978883.htmlAbout MarketsandMarkets™MarketsandMarkets™ provides quantified B2B research on 30,000 high growth niche opportunities/threats which will impact 70% to 80% of worldwide companies’ revenues.
Primary Cells Market Insights 2019, Global and Chinese Scenario is a professional and in-depth study on the current state of the global Primary Cells industry with a focus on the Chinese market.
The report provides key statistics on the market status of the Primary Cells manufacturers and is a valuable source of guidance and direction for companies and individuals interested in the industry.Overall, the report provides an in-depth insight of 2014–2024 global and Chinese Primary Cells market covering all important parameters.The key ponits of the report:The report provides a basic overview of the industry including its definition, applications and manufacturing technology.The report explores the international and Chinese major industry players in detail.
In this part, the report presents the company profile, product specifications, capacity, production value, and 2014–2019 market shares for each company.Through the statistical analysis, the report depicts the global and Chinese total market of Primary Cells industry including capacity, production, production value, cost/profit, supply/demand and Chinese import/export.The total market is further divided by company, by country, and by application/type for the competitive landscape analysis.The report then estimates 2019–2024 market development trends of Primary Cells industry.
Analysis of upstream raw materials, downstream demand, and current market dynamics is also carried out.The report makes some important proposals for a new project of Primary Cells Industry before evaluating its feasibility.Critical Questions Addressed by the Report What are the current trends that are driving the Primary Cellsmarket?Where will all these developments take the industry in the mid- to long-term?Who are the top vendors in the Primary Cellsmarket and what is their competitive analysis?How is adoption of Primary Cellssolutions among different industries?How is the adoption of Primary Cellssolutions among different regions?Report Sample includes:- Table of Contents- List of Tables & Figures- Charts- Research MethodologyGet FREE Sample of this Report at https://www.24marketreports.com/report-sample/global-primary-cells-2019-2024-665There are 3 key segments covered in this report: competitor segment, product type segment, end use/application segment.For competitor segment, the report includes global key players of Primary Cells as well as some small players.
At least 12 companies are included:LonzaThermo Fisher ScientificSigma-AldrichCell BiologicsPromoCell GmbHCurelineThe information for each competitor includes:Company ProfileMain Business InformationSWOT AnalysisSales, Revenue, Price and Gross MarginMarket ShareFor product type segment, this report listed main product type of Primary Cells market in gloabal and china.Human Primary CellsAnimal Primary CellsFor end use/application segment, this report focuses on the status and outlook for key applications.
End users sre also listed.MedicalSantific ResearchOtherReasons to Purchase this Report:Estimates 2019–2024 Primary Cells market development trends with the recent trends and SWOT analysisMarket dynamics scenario, along with growth opportunities of the market in the years to comeMarket segmentation analysis including qualitative and quantitative research incorporating the impact of economic and policy aspectsRegional and country level analysis integrating the demand and supply forces that are influencing the growth of the market.Market value (USD Million) and volume (Units Million) data for each segment and sub-segmentCompetitive landscape involving the market share of major players, along with the new projects and strategies adopted by players in the past five yearsComprehensive company profiles covering the product offerings, key financial information, recent developments, SWOT analysis, and strategies employed by the major market players1-year analyst support, along with the data support in excel format.Any special requirements about this report, please let us know and we can provide custom report.What is the regional structure of the market?
Major Growth Drivers:Market growth is driven by the rising prevalence of cancer, increasing cancer research, rising investments in regenerative medicine research, rising demand for monoclonal antibodies, and rapid growth in the biotechnology and biopharmaceutical industries.According to the new market research report “Primary Cells Market by Origin (Human Primary Cells, Animal Primary Cells), Type (Hematopoietic, Dermatocytes, Gastrointestinal, Hepatocytes, Lung, Renal, Musculoskeletal, Heart), End User, Region – Global Forecast to 2025“, published by MarketsandMarkets™, global primary cells market is projected to reach USD 1,613 million by 2025 from USD 970 million in 2020, at a CAGR of 10.7%.Exhaustive secondary research was carried out to collect information on the market, its peer markets, and its parent market.
This study involved four major activities in estimating the current size of the primary cells market.
The next step was to validate these findings, assumptions, and sizing with industry experts across the value chain through primary research.
Both top-down and bottom-up approaches were employed to estimate the complete market size.Expected Revenue Growth:[179 Pages Report] The global primary cells market is projected to reach USD 1,613 million by 2025 from USD 970 million in 2020, at a CAGR of 10.7%.COVID-19 Impact on Global Primary Cells MarketCOVID-19 is an infectious disease caused by the most recently discovered novel coronavirus.
Largely unknown before the outbreak began in Wuhan (China) in December 2019, COVID-19 has moved from a regional crisis to a global pandemic in just a matter of a few weeks.
With the World Health Organization (WHO) officially declaring the outbreak of COVID-19 a pandemic, a mix of established pharmaceutical and biopharmaceutical companies, as well as small startups, have stepped forward to develop treatments and vaccines that target the infection caused by the novel coronavirus.Browse and in-depth TOC on “Primary Cells Market”108 – Tables34 – Figures179 – PagesDownload PDF Brochure: https://www.marketsandmarkets.com/pdfdownloadNew.asp?id=32854960Based on origin, the primary cells market is segmented into human and animal primary cells.
