The protein in our body controls the metabolic processes and helps with normal body functioning. Yet, specific impairments can lead to misfolded or mutated protein production, disrupting the functioning. There is a supply of proteins used as replacements at times like these.
With the production market acquiring large scale demands to fulfil, there is a complex process associated behind. Before jumping on them, understanding the basics of recombinant human protein production and their steps is of higher importance to the industry. This article covers the basics of the recombinant concept of proteins and DNAs, with a brief on recombinant protein production.
What Is Recombinant DNA?
Proteins are the material that forms the body and performs the essential functions, and regulates performance. Digesting food with various digestive enzymes is that it breaks down macromolecules into smaller moles of food to be absorbed by the body. These enzymes are proteins.
Since the construction of protein is through DNA, two steps take place in this process. With the first being transcription, the mRNA is made from DNA. And followed step translation, the protein is made out of mRNA.
Thus, we understand that DNA provides the sequence instructions for creating a particular protein, saying the gene expression is the same as the protein expression creation from the process.
What Is A Recombinant Protein?
Recombinant proteins are made artificially through recombinant DNA technology. Such recombinant proteins are utilized in several applications in almost every industry, from cosmetics to research and development.
Isolating a particular protein naturally and delivering it to the market does not simply qualify to meet the ever-growing demand. Hence, recombinant DNA technology proves correct to provide large amounts of proteins sufficiently.
The recombinant DNA technology allows the manipulation of the protein properties of interest and creates the recombinant protein of choice. In scientific aspects, this technology is highly beneficial for developing recombinant proteins or human proteins for industrial needs.
Steps In The Production Of Recombinant Proteins
There was a domain of expert methodologies present when it came to the production of recombinant proteins. Yet, recombinant protein production has become a widespread technique that has made the development adaptable and straightforward to all the commercially available systems.
To the recombinant protein production process, there are varied questions that must be considered and later adapted to the most optimal methodology.
- Which system should be utilized for expressing the protein?
- Which expression vector will be the best?
- Should protein expression be in full length or partially?
- Should the protein be tagged?
- How to purify the protein created?
With the above questions, there are a lot of significant decisions made while opting for the production of recombinant human proteins. The choice must be taken depending on the protein of interest.
The most suitable methodology used over the past few decades for producing large units of recombinant protein from different organisms is through recombinant DNA technology. The purifications are taken care of in the lab, and the process summarizes with the following steps:
- Obtaining the cDNA and creating the expression clone
- Cloning
- Expressing the protein in a suitable system
- Small-scale test expression
- Protein purification
- Protein characterization
There are many systems for expressing the recombinant human protein, and they include two different types of systems: cell-based and cell-free.
Take Away
Many factors influence the expression of recombinant proteins, and there is a growing demand for the production of the same. With them being produced too fast, the protein of interest is developed with the mature technique of recombinant protein production. There are several systems available for production and purification.
We at Genext Genomics are one such catering domain serving the industry demands for the recombinant human protein. Our high-quality recombinant proteins are produced with the latest suitable technology and delivered to the growing needs.
With our team of professionals and experienced scientists, we cater to commercial systems and overcome the challenges of successful protein production and purification. We take pride in serving the research and development industry with competitive market prices.
A new report published by Fact.MR estimates the global recombinant protein market to witness expansion at a moderate CAGR during the forecast period 2017 to 2022.
Revenues from the global recombinant protein market will reach nearly US$ 460 Mn by 2022-end.Request for the Report Summary: https://www.factmr.com/report/186/recombinant-protein-marketThrough genetic engineering, and cell engineering, recombinant protein are produced from creature body.
Becoming the new standard, this technology is witnessing a gradual shift from bulk production procedure towards post-production techniques, which in turn promises an enhanced delivery and stability.
However, blood coagulation factor is excluded, as it possesses an extremely limited source.
This is mainly to compensate functional proteins in vivo defects, and for increasing the functions of proteins in a body.
These factors provide recombinant proteins with substantially higher safety as compared to small molecules, further leading towards higher approval rates.The clinical trial periods for recombinant proteins are comparatively shorter than small molecule drugs, which further aids in enhancing patient protection, and renders pharmaceutical companies with a relatively longer exclusive sales time.
