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UV-VIS Spectrophotometers

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Labozon Scientific Inc
UV-VIS Spectrophotometers

UV-VIS spectrophotometers are analytical instruments used to measure the absorption and transmission of light in the ultraviolet (UV) and visible (VIS) regions of the electromagnetic spectrum. These instruments are widely employed in various fields such as chemistry, biochemistry, pharmaceuticals, environmental science, and materials science.


The basic components of a UV-VIS spectrophotometer include a light source, a monochromator, a sample holder, a detector, and a data processing unit. The light source emits a broad spectrum of light, which is typically a combination of visible and UV light. Common light sources include tungsten-halogen lamps for the visible range and deuterium lamps for the UV range. Some advanced spectrophotometers may also utilize xenon or LED light sources.


The monochromator acts as a wavelength selector, allowing the user to choose a specific wavelength or a range of wavelengths to analyze. It consists of a prism or a diffraction grating that disperses the light into its constituent wavelengths. The desired wavelength is then selected by adjusting the position of the prism or grating. This monochromatic light is then directed toward the sample.


The sample holder, often a cuvette or a test tube, holds the sample in a fixed path length, ensuring consistent and reproducible measurements. The sample is placed between the light source and the detector, and its interaction with the incident light is measured.

UV-VIS Spectrophotometers are Equipped with a microprocessor to set parameters and a 1200-line double beam grating, which ensures high resolution. For more, visit www.Labozon.com

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